Molecular cloning and sequence analysis of human preprocathepsin C

FEBS Letters

Pungerčar, Jože¹  Dolenc, Iztok¹  Pariš, Alenka¹  Renko, Metka¹  Štrukelj, Borut¹  Turk, Vito¹ 

[1] Department of Biochemistry and Molecular Biology, Jožef Stefan Institute, Jamova 39, 61000 Ljubljana, Slovenia

关键词: Preprocathepsin C;    Ileum (human);    cDNA cloning;    Cysteine proteinase;   

DOI  :  10.1016/0014-5793(95)00777-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A cDNA clone (C1) coding for human preprocathepsin C was isolated from a human ileum cDNA library using a rat kidney-derived RT-PCR probe and its complete nucleotide sequence determined. The full-length 1857 bp sequence codes for a protein of 463 amino acid residues with a calculated molecular mass of 51848 Da. Comparison of the deduced amino acid sequence with that of rat preprocathepsin C indicates an 87.5% identity. A multiple alignment of the deduced cathepsin C sequence of 233 residues which, by analogy to other cysteine proteinases, corresponds to the mature protein, confirms that human cathepsin C belongs to the papain superfamily.

【 授权许可】

Unknown