Molecular cloning of rat GADD45γ, gene induction and its role during neuronal cell death

FEBS Letters

Suzuki, Harukazu¹  Mayumi-Matsuda, Keiko¹  Sakata, Tsuneaki¹  Kojima, Satoshi¹ 

[1] Shionogi Institute for Medical Science, 2-5-1, Mishima, Settsu-shi, Osaka 566-0022, Japan

关键词: Restriction landmark cDNA scanning;    Microinjection;    GADD;    growth arrest and DNA damage-induced protein;    NGF;    nerve growth factor;    SCG;    superior cervical ganglia;   

DOI  :  10.1016/S0014-5793(99)00234-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

To study the molecular mechanism of neuronal cell death, we carried out the screening of genes which were induced during the neuronal cell death of neuronal PC12. We cloned the cDNA of rat GADD45γ, the third member of the GADD45 family. Induction of GADD45γ mRNA was observed in the neuronal cell death caused by depletion of neurotrophic factor and Ca²⁺ ionophore treatment. Overexpression of GADD45γ in superior cervical ganglion neurons caused cell death. These results suggest that GADD45γ plays an important role in neuronal cell death.

【 授权许可】

Unknown