After microinjection hemimethylated DNA is converted into symmetrically methylated DNA before DNA replication

FEBS Letters

Graessmann, A.¹  Guhl, E.¹  Sandberg, G.¹  Graessmann, M.¹ 

[1] Institut für Molekularbiologie und Biochemie der Freien Universität Berlin, Arnirnallee 22, 1 Berlin 33, Germany

关键词: Hemimethylated DNA;    Microinjection;    Maintenance methylation;    DNA replication;    5-Azacytidine;   

DOI  :  10.1016/0014-5793(91)80599-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In this investigation we analysed the maintenance methylation activity of the mammalian cell DNA methyltransferase by microinjection of hemimethylated HSV-tk DNA into thymidine kinase-negative rat 2 cells. We found that the hemimethylated DNA was efficiently converted into symmetrical methylated molecules before DNA replication. Furthermore, integration of the trans-DNA into the host genome is an early event after gene transfer.

【 授权许可】

Unknown