αB‐crystallin in the rat lens is phosphorylated at an early post‐natal age

FEBS Letters

Kato, Kanefusa¹  Iwamoto, Ikuko¹  Kamei, Keiko¹  Inaguma, Yutaka¹  Iida, Kayo¹  Ito, Hidenori¹ 

[1] Department of Biochemistry, Institute for Developmental Research, Aichi Human Service Center, Kasugai, Aichi 480-0392, Japan

关键词: Lens;    Crystallin;    Phosphorylation;    MAP kinase;   

DOI  :  10.1016/S0014-5793(99)00242-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We determined the developmental changes in the phosphorylation state of αB-crystallin in lenses from rats at various post-natal ages by isoelectric focusing gel electrophoresis or sodium dodecyl sulfate-polyacrylamide gel electrophoresis and a subsequent Western blot analysis of extracts of lenses using antibodies that recognized the carboxy-terminal sequence or each of the three phosphorylated serine residues (Ser-19, Ser-45 and Ser-59) in αB-crystallin. Phosphorylated forms of αB-crystallin were barely detected at birth but they became detectable at 3 weeks of age and reached plateau levels at 8 weeks of age. The phosphorylation of αB-crystallin at Ser-45 was observed preferentially. The active form of p44/42 MAP kinase, which is responsible for the phosphorylation of Ser-45 in αB-crystallin, also increased in a development-dependent manner. Thus we found that the developmental increase of the phosphorylation at Ser-45 of αB-crystallin in the rat lens was due to the developmental activation of p44/42 MAP kinase.

【 授权许可】

Unknown