The chum salmon IGF‐II gene promoter is activated by hepatocyte nuclear factor 3β

FEBS Letters

Sussenbach, John S.¹  Palamarchuk, Alexey Y.¹  Holthuizen, P.Elly¹  Kavsan, Vadim M.² 

[1] Laboratory for Physiological Chemistry, Utrecht University, Universiteitsweg 100, 3584 CG Utrecht, The Netherlands;Department of Biosynthesis of Nucleic Acids, Institute of Molecular Biology and Genetics, National Academy of Sciences of the Ukraine, Zabolotnogo St. 150, 252627 Kiev, Ukraine

关键词: Insulin-like growth factor II;    Gene expression;    Hepatocyte nuclear factor 3β;    Salmon;    Oncorhynchus keta;    bp;    base pair;    IGF-II;    insulin-like growth factor II;    sIGF-II;    chum salmon IGF-II;    HNF-3β;    hepatocyte nuclear factor 3β;    5′-UTR;    5′-untranslated region;   

DOI  :  10.1016/S0014-5793(99)00219-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

IGF-II plays an important role in growth and development of vertebrates and is highly expressed in adult salmon liver. In the present study, we demonstrate that a liver-enriched transcription factor, hepatocyte nuclear factor 3β (HNF-3β), is an activator of the chum salmon IGF-II gene. Multiple binding sites for HNF-3β were identified within the 5′-UTR using electrophoretic mobility shift assays and mutation of these sites prevents binding of HNF-3β. In transient transfection assays it was shown that mutation of the HNF-3β binding sites results in a substantial decrease of HNF-3β-activated salmon IGF-II gene expression. This is the first identified transcription factor that is functionally involved in the regulation of fish IGF-II expression.

【 授权许可】

Unknown