Organization and expression of the chum salmon insulin‐like growth factor II gene

FEBS Letters

Sussenbach, John S.³  Müller, Werner E.G.¹  Palamarchuk, Alexey Y.²  Holthuizen, P.Elly³  Kavsan, Vadim M.² 

[1] Institute for Physiological Chemistry, University of Mainz, Duesbergweg 6, 55099 Mainz, Germany;Department of Biosynthesis of Nucleic Acids, Institute of Molecular Biology and Genetics, National Academy of Sciences of the Ukraine, 150, Zabolotnogo St., 252627 Kiev, Ukraine;Laboratory for Physiological Chemistry, University of Utrecht, P.O. Box 80042, 3508 TA Utrecht, The Netherlands

关键词: Insulin-like growth factor II;    Gene structure;    Gene expression;    Fish;    Salmon;    Oncorhynchus keta;    nt;    nucleotide;    bp;    base pair;    kbp;    kilobase pair;    aa;    amino acid;    RT-PCR;    coupled reverse transcription-polymerase chain reaction;    IGF-II;    insulin-like growth factor II;    sIGF-II;    chum salmon IGF-II;    UTR;    untranslated region;    SDS;    sodium dodecyl sulfate;    SSC;    standard sodium chloride/sodium citrate buffer;   

DOI  :  10.1016/S0014-5793(97)01217-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

IGF-II plays an important role in growth and development of vertebrates. In the present study, the characterization of the first fish IGF-II gene, chum salmon IGF-II, is described. The sIGF-II gene consists of four exons, spanning a region of 9 kbp, that encode the 214 aa IGF-II precursor. While the amino acid sequences of fully processed IGF-II of salmon and mammalian species are very similar, the prepro-peptide sequence deviates extensively in the signal- and E-peptide domains. The transcription initiation site of the sIGF-II gene was localized within a 30 nt region employing RT-PCR. Using sIGF-II promoter-luciferase constructs it was demonstrated that the sIGF-II gene has a relatively strong promoter that contains tissue-specific regulatory elements.

【 授权许可】

Unknown