Role of ERK1/ERK2 and p70S6K pathway in insulin signalling of protein synthesis

FEBS Letters

Arnott, Caroline H.¹  Sale, Elizabeth M.¹  Atkinson, Peter P.G.¹  Chad, John E.¹  Sale, Graham J.¹ 

[1] Division of Biochemistry and Molecular Biology, School of Biological Sciences, Biomedical Sciences Building, Bassett Crescent East, Southampton SO16 7PX, UK

关键词: Insulin;    Protein synthesis;    Extracellular signal regulated kinase 1;    Extracellular signal regulated kinase 2;    p70S6k;    Antisense;    ERK1/ERK2;    extracellular signal regulated kinases 1 and 2 (also known as p44 and p42 MAP kinase);   

DOI  :  10.1016/S0014-5793(99)00193-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The signalling pathways by which insulin triggers protein synthesis were studied using an antisense strategy to deplete ERK1/ERK2 and rapamycin to inhibit the p70^S6K pathway. The results indicated that ERK1/ERK2 principally regulated the amount of the protein synthesis machinery available in the cell while the p70^S6K pathway contributed to modulating its activation in response to insulin. ERK1/ERK2 also mediated in a small proportion of insulin-stimulated protein synthesis which included the induction of c-fos protein. When c-fos induction was blocked the majority of insulin-stimulated protein synthesis still occurred and thus did not require transcriptional regulation of c-fos or its targets.

【 授权许可】

Unknown