A positive relationship between protein synthetic rate and intracellular glutamine concentration in perfused rat skeletal muscle

FEBS Letters

MacLennan, Peter A.¹  Rennie, Michael J.¹  Brown, R.A.² 

[1] Department of Physiology, University of Dundee, Dundee DD1 4HN, Scotland;Department of Mathematics, University of Dundee, Dundee DD1 4HN, Scotland

关键词: Glutamine;    Protein synthesis;    Insulin;    (Muscle;    Injury);   

DOI  :  10.1016/0014-5793(87)80139-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

During muscle-protein wasting associated with injury and disease the distribution ratio of free glutamine between muscle and blood falls. In pursuing possible consequences of this, we investigated the relationship between the rate of muscle protein synthesis and intramuscular glutamine concentration, manipulated acutely in the isolated perfused rat hindquarter. Increasing perfusate glutamine from 0.67 to 5.0 mM caused a 200% increase in intracellular glutamine and a 66% increase in protein synthesis in the absence of insulin; in the presence of insulin a 30% increase in intramuscular glutamine was accompanied by an 80% increase in protein synthesis. Analysis of variance of the results confirmed the existence of positive relationships between intramuscular glutamine and protein synthesis in the presence or absence of insulin. Control of the size of the intramuscular free pool of glutamine may be important in determining the muscle protein mass.

【 授权许可】

Unknown