Discovery of a receptor related to the galanin receptors

FEBS Letters

Howard, Andrew D¹  Tan, Carina P¹  George, Susan R⁴  O'Neill, Gary P²  Coulombe, Nathalie²  Nguyen, Tuan³  Cheng, Regina³  Liu, Yang⁴  Lee, Dennis K⁴  O'Dowd, Brian F⁴  Tang-Nguyen, Anh-Thi⁴ 

[1] Department of Biochemistry and Physiology, Merck Research Laboratories, Rahway, NJ 07065, USA;Department of Biochemistry and Molecular Biology, Merck-Frosst Center for Therapeutic Research, 16711 TransCanada Highway, Kirkland, Que. H9H 3L1, Canada;Centre for Addiction and Mental Health, 33 Russell Street, Toronto, Ont. M5S 2S1, Canada;Department of Pharmacology, University of Toronto, Medical Sciences Building, Toronto, Ont. M5S 1A8, Canada

关键词: G protein-coupled receptor;    Polymerase chain reaction;    Central nervous system expression;    Chromosome;   

DOI  :  10.1016/S0014-5793(99)00009-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We report the isolation of a cDNA clone named GPR54, which encodes a novel G protein-coupled receptor (GPCR). A PCR search of rat brain cDNA retrieved a clone partially encoding a GPCR. In a library screening this clone was used to isolate a cDNA with an open reading frame (ORF) encoding a receptor of 396 amino acids long which shared significant identities in the transmembrane regions with rat galanin receptors GalR1 (45%), GalR3 (45%) and GalR2 (44%). Northern blot and in situ hybridization analyses revealed that GPR54 is expressed in brain regions (pons, midbrain, thalamus, hypothalamus, hippocampus, amygdala, cortex, frontal cortex, and striatum) as well as peripheral regions (liver and intestine). In COS cell expression of GPR54 no specific binding was observed for ¹²⁵I-galanin. A recent BLAST search with the rat GPR54 ORF nucleotide sequence recovered the human orthologue of GPR54 in a 3.5 Mb contig localized to chromosome 19p13.3.

【 授权许可】

Unknown