Characterization of a human gene related to genes encoding somatostatin receptors

FEBS Letters

Johnson, Michael P.⁴  Lynch, Kevin R.¹  Kolakowski, Lee F.⁴  Nguyen, Tuan⁵  Cheng, Regina³  George, Susan R.⁵  Jung, Benjamin P.⁵  Heng, Henry H.Q.²  O'Dowd, Brian F.⁵ 

[1] Department of Pharmacology, University of Virginia, Health Sciences Center, 1300 Jefferson Park Avenue, Charlottesville, VA 22908, USA;SeeDNA Biotech Inc., Farquharson Bldg, 4700 Keele Street, Downsview, Ontario M3J 1P3, Canada;Addiction Research Foundation, 33 Russell Street, Toronto, Ontario M5S 2S1, Canada;Department of Pharmacology, University of Texas Health Science Center at San Antonio, San Antonio, Texas, 78284, USA;Department of Pharmacology, University of Toronto, Toronto, Ontario, M5S 1A8, Canada

关键词: Polymerase chain reaction;    Chromosome;    Northern blot;    Intronless;   

DOI  :  10.1016/S0014-5793(96)01160-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We report the identification of a gene, named SLC-1 ¹, encoding a novel G protein-coupled receptor (GPCR). A customized search procedure of a database of expressed sequence tags (dbEST) retrieved a human cDNA sequence that partially encoded a GPCR. A genomic DNA fragment identical to the cDNA was obtained and used to screen a library to isolate the full-length coding region of the gene. This gene was intronless in its open reading frame, and encoded a receptor of 402 amino acids, and shared −40% amino acid identity in the transmembrane (TM) regions to the five known human somatostatin receptors. Northern blot analysis revealed that SLC-1 is expressed in human brain regions, including the forebrain and hypothalamus. Expression in the rat was highest in brain, followed by heart, kidney, and ovary. Expression of SLC-1 in COS-7 cells failed to show specific binding to radiolabelled Tyr¹-somatostatin-14, naloxone, bremazocine, 1,3-di(2-tolyl)-guanidine (DTG), or haloperidol. A repeat polymorphism of the form (CA) _n was discovered in the 5′-untranslated region (UTR) of the gene and SLC-1 was mapped to chromosome 22, q13.3.

【 授权许可】

Unknown