Carboxyatractyloside increases the effect of oleate on mitochondrial permeability transition

FEBS Letters

Chávez, Edmundo¹  Zazueta, Cecilia¹  Garcı́a, Noemı́¹ 

[1] Departamento de Bioquı́mica, Instituto Nacional de Cardiologı́a, Ignacio Chávez, Juan Badiano #1, Col. Secc. XVI, Mexico D.F. 014080, Mexico

关键词: Permeability transition;    Fatty acid;    Carboxyatractyloside;    Mitochondrion;    ADP/ATP carrier;    Calcium efflux;    CAT;    carboxyatractyloside;    CSA;    cyclosporin A;    ΔΨ;    transmembrane potential;    ANT;    adenine nucleotide translocase;    FCCP;    p-trifluoromethoxycarbonylcyanide phenylhydrazone;    NEM;    N-ethylmaleimide;   

DOI  :  10.1016/S0014-5793(99)00128-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Addition of a low concentration of carboxyatractyloside (0.075 μM) renders mitochondria susceptible to the opening of the non-specific pore by 5 μM oleate, in a cyclosporin A-sensitive fashion. Matrix Ca²⁺ efflux as well as collapse of the transmembrane potential reveal permeability transition. The effect of oleate is reached after the titration, by carboxyatractyloside, of 38 pmol of adenine nucleotide translocase per mg mitochondrial protein. We propose that permeability transition may result from an additive action of carboxyatractyloside plus oleate on the ADP/ATP carrier.

【 授权许可】

Unknown