The permeability transition pore induced under anaerobic conditions in mitochondria energized with ATP

FEBS Letters

Zhuravlyova, Anastasiya V¹  Zorov, Dmitry B²  Vyssokikh, Mikhail Yu²  Krasnikov, Boris F²  Zorova, Ljubava D³  Kuzminova, Alevtina E² 

[1] Chemical Faculty, Moscow State University, Moscow, Russia;A.N. Belozersky Institute of Physico-Chemical Biology, Moscow State University, Moscow, Russia;Biological Faculty, Moscow State University, Moscow, Russia

关键词: Mitochondrion;    Permeability transition;    Oxygen;    Hypoxia;    PTP;    permeability transition pore;    ROS;    reactive oxygen species;    PEP;    phosphoenolpyruvate;    PK;    pyruvate kinase;   

DOI  :  10.1016/S0014-5793(98)01005-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The role of oxygen in the induction of mitochondrial permeability transitions was studied. Oxygen consumption, swelling, membrane potential and calcium transport were recorded simultaneously in isolated rat liver mitochondria. Oxygen depletion was accomplished by saturating the medium with N₂ and allowing either mitochondrial respiration or glucose/glucose oxidase to consume the residual oxygen. Upon anaerobiosis, mitochondria were supplemented with 500 μM ATP to support succinate-driven membrane potential. Under these conditions, 100 μM Ca²⁺ induced cyclosporin A-sensitive permeability transitions. To eliminate the possible inhibition of permeability transition by high concentrations of adenine nucleotides, anaerobic mitochondria were also energized by the combination of 20 μM ADP and phosphoenolpyruvate/pyruvate kinase. These mitochondria also underwent Ca²⁺-induced permeability transition. Under both of these conditions, namely the addition of ATP as a single or through actions of pyruvate kinase, the respiratory components were totally reduced. Thus, oxygen is not a necessary factor for mitochondria to undergo permeability transitions.

【 授权许可】

Unknown