Trans‐splicing of a voltage‐gated sodium channel is regulated by nerve growth factor

FEBS Letters

Souslova, Veronika²  Akopian, Armen N.²  England, Steven²  Ogata, Nobukino¹  Wood, John N.²  Okuse, Kenji² 

[1] Second Department of Physiology, Faculty of Medicine, Hiroshima University, Hiroshima 734, Japan;Department of Biology, University College, Gower Street, London WC1E 6BT, UK

关键词: Trans-splicing nerve growth factor;    Sensory neuron specific;    dorsal root ganglia;    DRG;    dorsal root ganglia;    NGF;    nerve growth factor;    SNS;    sensory neuron specific;    TTXi;    tetrodotoxin-insensitive;    TTXs;    tetrodotoxin-sensitive;   

DOI  :  10.1016/S0014-5793(99)00126-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Mammalian sensory neurons express a voltage-gated sodium channel named SNS. Here we report the identification of an SNS transcript (SNS-A) that contains an exact repeat of exons 12, 13 and 14 encoding a partial repeat of domain II. Because the exons 12–14 are present in single copies in genomic DNA, the SNS-A transcript must arise by trans-splicing. Nerve growth factor, which regulates pain thresholds, and the functional expression of voltage-gated sodium channels increases the levels of the SNS-A transcript several-fold both in vivo and in vitro as measured by RNase protection methods, as well as RT-PCR. These data demonstrate a novel regulatory role for the nerve growth factor and are the first example of trans-splicing in the vertebrate nervous system.

【 授权许可】

Unknown