Overexpression of nucleoside diphosphate kinases induces neurite outgrowth and their substitution to inactive forms leads to suppression of nerve growth factor‐ and dibutyryl cyclic AMP‐induced effects in PC12D cells

FEBS Letters

Ishijima, Yasushi¹  Kimura, Narimichi¹  Yamada, Takeshi²  Miyazaki, Hidetaka¹  Shimada, Nobuko¹  Fukuda, Mitsugu¹  Orlova, Tatiana G¹  Orlov, Nicolay Ya¹ 

[1]Department of Gene Regulation and Protein Function, Tokyo Metropolitan Institute of Gerontology, 35-2 Sakaecho, Itabashi-ku, Tokyo 173-0015, Japan

[2]Department of Biomolecular Science, Faculty of Science, Toho University, 2-2-1 Miyama, Funabashi, Chiba 274-8510, Japan

关键词: Nucleoside diphosphate kinase;    Enzyme activity;    Neurite outgrowth;    NGF;    Cyclic AMP;    PC12 cell;    NDPK;    nucleoside diphosphate kinase;    NGF;    nerve growth factor;    dbcAMP;    dibutyryl cyclic AMP;    HA;    hemagglutinin;    PCR;    polymerase chain reaction;    IPTG;    isopropyl-β-d-thiogalactopyranoside;    PMSF;    phenylmethanesulfonyl fluoride;    BSA;    bovine serum albumin;    PBS;    Dulbecco's phosphate-buffered saline;   

DOI  :  10.1016/S0014-5793(99)00116-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Whether nucleoside diphosphate kinase (NDPK) is involved in neuronal differentiation was investigated with special reference to its enzyme activity. Neurite outgrowth of PC12D cells induced by nerve growth factor or a cyclic AMP analog was suppressed to some extent when inactive NDPKs (the active site histidine 118 was replaced with alanine), not active forms, were transiently overexpressed. This suppression was more definite in their stably expressed clones. NDPKβ-transfected clones and, to a lesser extent, NDPKα-transfected clones, but not inactive NDPK-transfected clones, extended neurites without differentiation inducers. These results imply that NDPKs may play a role by exerting their enzyme activity during differentiation of PC12 cells.

【 授权许可】

Unknown