Inhibition of nerve growth factor‐induced neurite outgrowth of PC12 cells by a protein kinase inhibitor which does not permeate the cell membrane

FEBS Letters

Matsuda, Yuzuru¹  Nakanishi, Satoshi¹  Nagashima, Ken¹ 

[1] Tokyo Research Laboratories, Kyowa Hakko Kogyo Co. Ltd., 3-6-6 Asahimachi, Machida-shi, Tokyo 194, Japan

关键词: Nerve growth factor;    Neurite outgrowth;    PC12;    K-252a;    K-252b;    Ecto-protein kinase;    NGF;    nerve growth factor;    HEPES;    4-(2-hudroxyethyl)-1-piperazineethanesulfonic acid;    dibutyryl cAMP;    N-2′-dibutyryladenosine 3′;    5′-cyclic monophosphate;   

DOI  :  10.1016/0014-5793(91)81165-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

K-252a, a protein kinase inhibitor of microbial origin, has proven to be a specific inhibitor of nerve growth factor. In this study, the effects of K-252b, the 9-carboxylic acid derivative of K-252a, on nerve growth factor-induced neurite outgrowth in PC12 cells was examined. K-252b is hydrophilic and does not permeate the cell membrane of PC12 cells, whereas K-252a clearly does. K-252b is, however, as potent as K-252a itself in inhibiting the nerve growth factor-induced neurite outgrowth. These results can be interpreted to suggest that effects of K-252b may be through surface-bound/anchored K-252b-sensitive molecules on PC12 cells.

【 授权许可】

Unknown