Neurite outgrowth from PC12 cells is enhanced by an inhibitor of mechanical channels

NEUROSCIENCE LETTERS

卷:481

Article

Gottlieb, Philip A.²  Barone, Tara¹  Plunkett, Robert^1,2 

[1] Roswell Pk Canc Inst, Dept Neurosurg, Buffalo, NY 14263 USA

[2] SUNY Buffalo, Dept Neurosurg, Buffalo, NY 14214 USA

关键词: Neurite outgrowth;    Mechanical channels;    GsMTx4;    Peptide inhibitor;    NGF;    PC12;   

DOI  :  10.1016/j.neulet.2010.06.066

来源: Elsevier

【 摘 要 】

GsMTx4, a peptide inhibitor for mechanosensitive ion channels (MSCs), promoted neurite outgrowth from PC12 cells in the presence of NGF in a dose-dependent manner between 5 and 10011,M peptide. Enhanced neurite growth required >12 h of peptide exposure in cells grown with NGF. Adsorption of GsMTx4 to serum proteins in the media lowered the free peptide concentration of 100 mu M to a free concentration of 5 mu M, a concentration shown to completely inhibit MSCs in the patch clamp assay. Outside-out patches from PC12 cells grown in NGF had mechanically activated cation channels that were reversibly inhibited by GsMTx4. These results are similar to those observed by Gomez and co-workers [4] in Xenopus spinal cord. The inhibition of mechanosensitive channels by GsMTx4 may be a useful approach to accelerate regeneration of neurons in neurodegenerative diseases and spinal cord injury. (C) 2010 Elsevier Ireland Ltd. All rights reserved.

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