Rho‐kinase in human neutrophils: a role in signalling for myosin light chain phosphorylation and cell migration

FEBS Letters

Niggli, Verena¹ 

[1] Department of Pathology, University of Bern, Murtenstr. 31, P.O. Box 62, CH-3010 Bern, Switzerland

关键词: Rho-associated coiled-coil forming kinase;    Cell migration;    Myosin light chain;    Signal transduction;    Human neutrophil;    fNLPNTL;    N-formyl-l-norleucyl-l-leucyl-l-phenylalanyl-l-norleucyl-l-tyrosyl-l-lysine;    HSA;    human serum albumin;    MLC;    myosin light chain;    ROCK;    Rho-associated coiled-coil forming kinase;   

DOI  :  10.1016/S0014-5793(99)00098-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The role of a Rho-associated coiled-coil forming kinase in migration of neutrophils has been investigated. Rho-associated coiled-coil forming kinase I was expressed in human neutrophils. Chemotactic peptide led to a Rho-associated coiled-coil forming kinase-dependent increase in phosphorylation of myosin light chain. This was determined with the help of an antibody directed against serine 19-phosphorylated myosin light chain and an inhibitor of Rho-associated coiled-coil forming kinase (Y-27632). Y-27632 suppressed myosin light chain phosphorylation and chemotactic peptide-induced development of cell polarity and locomotion with similar potency (ED₅₀ 0.5–1.1 μM). The data strongly suggest that a Rho-associated coiled-coil forming kinase isoform, activated in human neutrophils exposed to chemotactic peptide, is important for motile functions of these cells.

【 授权许可】

Unknown