ROCK‐I and ROCK‐II, two isoforms of Rho‐associated coiled‐coil forming protein serine/threonine kinase in mice

FEBS Letters

Fujisawa, Kazuko¹  Ishizaki, Toshimasa¹  Nakagawa, Osamu¹  Nakao, Kazuwa²  Saito, Yuji¹  Narumiya, Shuh¹ 

[1]Department of Pharmacology, Kyoto University Faculty of Medicine, Sakyo-ku, Kyoto 606, Japan

[2]Department of Medicine and Clinical Science, Kyoto University Faculty of Medicine, Sakyo-ku, Kyoto 606, Japan

关键词: Rho GTPase;    Signal transduction;    Protein serine/threonine kinase;    cDNA cloning;    Gene expression;    ROCK;    Rho-associated coiled-coil forming kinase;    GTP;    guanosine 5′-triphosphate;    GDP;    guanosine 5′-diphosphate;    GTPγS;    guanosine 5′-(γ-thio)triphosphate;    MD-PK;    myotonic dystrophy protein kinase;    GDPβS;    guanosine 5′-(β-thio)diphosphate;    GAPDH;    glyceraldehyde-3-phosphate dehydrogenase;   

DOI  :  10.1016/0014-5793(96)00811-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We recently identified a novel human protein kinase, p160 ROCK, as a putative downstream target of the small GTPase Rho. Using the human ROCK cDNA as a probe, we isolated cDNA of two distinct, highly related sequences from mouse libraries. One encoded a mouse counterpart of human ROCK (ROCK-I), and the other encoded a novel ROCK-related kinase (ROCK-II). Like ROCK/ROCK-I, ROCK-II also bound to GTP-Rho selectively. ROCK-I mRNA was ubiquitously expressed except in the brain and muscle, whereas ROCK-II mRNA was expressed abundantly in the brain, muscle, heart, lung and placenta. These results suggest that at least two ROCK isoforms are present in a single species and play distinct roles in Rho-mediated signalling pathways.

【 授权许可】

Unknown