Cloning of a novel kinase (SIK) of the SNF1/AMPK family from high salt diet‐treated rat adrenal1

FEBS Letters

Nonaka, Yasuki¹  Takemori, Hiroshi¹  Wang, Zhi-nong¹  Halder, Sunil K¹  Okamoto, Mitsuhiro¹ 

[1]Department of Molecular Physiological Chemistry, Osaka University Medical School, 2-2 Yamadaoka, Suita, Osaka 565-0871, Japan

关键词: Salt inducible kinase;    Protein serine/threonine kinase;    SNF1/AMPK;    Adrenocorticotropic hormone;    Y1 mouse adrenocortical tumor cell;    Adrenocortical function;    AII;    angiotensin II;    ACTH;    adrenocorticotropic hormone;    SIK;    salt-inducible kinase;    SNF1;    sucrose-non-fermenting 1 protein kinase;    AMPK;    AMP-activated protein kinase;    RACE;    rapid amplification of cDNA ends;    PCR;    polymerase chain reaction;    G3PDH;    glyceraldehyde 3-phosphate dehydrogenase;    GST;    glutathione S-transferase;    SDS-PAGE;    sodium dodecyl sulfate-polyacrylamide gel electrophoresis;    PKA;    cAMP-dependent protein kinase;    PKC;    calcium/phospholipid-dependent protein kinase;   

DOI  :  10.1016/S0014-5793(99)00708-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

PCR-coupled cDNA subtraction hybridization was adapted to identify the genes expressed in the adrenocortical tissues from high salt diet-treated rat. A novel cDNA clone, termed alt-nducible inase (SIK), encoding a polypeptide (776 amino acids) with significant similarity to protein serine/threonine kinases in the SNF1/AMPK family was isolated. An in vitro kinase assay demonstrated that SIK protein had autophosphorylation activity. Northern blot revealed that SIK mRNA levels were markedly augmented by ACTH treatment both in rat adrenal glands and in Y1 cells. SIK may play an important role in the regulation of adrenocortical functions in response to high plasma salt and ACTH stimulation.

【 授权许可】

Unknown