Calcitonin gene‐related peptide decreases expression of acetylcholinesterase in mammalian myotubes

FEBS Letters

Boudreau-Larivière, Céline¹  Jasmin, Bernard J¹ 

[1] Department of Cellular and Molecular Medicine, Faculty of Medicine, University of Ottawa, 451 Smyth Road, Ottawa, Ont. K1H 8M5, Canada

关键词: Neuromuscular junction;    Trophic factor;    Synaptic protein;    Skeletal muscle;   

DOI  :  10.1016/S0014-5793(99)00015-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Nerve-derived trophic factors are known to modulate expression of acetylcholinesterase (AChE) in skeletal muscle fibers, yet the precise identity of these factors remains elusive. In the present study, we treated mouse C2 myotubes with calcitonin gene-related peptide (CGRP). Compared to non-treated myotubes, cell-associated AChE activity levels were decreased by ∼60% after 48 h of treatment. A parallel reduction in AChE total protein levels was also observed as determined by Western blot analysis. The reduction in AChE activity was due to a decrease in the levels of the G₁ molecular form and to an elimination of G₄. By contrast, levels of secreted AChE remained unchanged following CGRP treatment. Finally, the overall decrease in AChE activity was accompanied by a reduction in AChE transcripts which could not be attributed to changes in the transcriptional rate of the ACHE gene.

【 授权许可】

Unknown