Molecular cloning and characterization of a novel angiopoietin family protein, angiopoietin‐3

FEBS Letters

Ahn, Ji Eun¹  Koh, Gou Young¹  Kim, Injune¹  Liu, Mingzhu¹  So, June-No²  Koh, Keum Nim¹  Kwak, Hee Jin¹ 

[1] National Creative Research Initiatives Center for Cardiac Regeneration and Institute of Cardiovascular Research, Chonbuk National University School of Medicine, San 2-20, Keum-Am-Dong, Chonju 560-180, South Korea;Department of Biotechnology, Woosuk University, Chonju, South Korea

关键词: Angiopoietin;    Endothelial cell;    Vasculogenesis;    PCR;    polymerase chain reaction;    CMV;    cytomegalovirus;    cDNA;    complementary deoxyribonucleic acid;   

DOI  :  10.1016/S0014-5793(99)00008-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Using homology-based PCR, we have isolated cDNA encoding a novel member (491 amino acids) of the angiopoietin (Ang) family from human adult heart cDNA and have designated it angiopoietin-3 (Ang3). The NH₂-terminal and COOH-terminal portions of Ang-3 contain the characteristic coiled-coil domain and fibrinogen-like domain that are conserved in other known Angs. Ang3 has a highly hydrophobic region at the N-terminus (∼21 amino acids) that is typical of a signal sequence for protein secretion. Ang3 mRNA is most abundant in adrenal gland, placenta, thyroid gland, heart and small intestine in human adult tissues. Additionally, Ang3 is a secretory protein, but is not a mitogen in endothelial cells.

【 授权许可】

Unknown