Cloning of a bovine orphan transporter and its short splicing variant

FEBS Letters

Shimada, Shoichi¹  Inoue, Kiyoshi²  Tohyama, Masaya²  Sakata, Kazuko²  Yamashita, Toshihide² 

[1] Second Department of Anatomy, Nagoya City University Medical School, 1 Kawasumi Mizuho, Mizuho, Nagoya 467-0001, Japan;Department of Anatomy and Neuroscience, Osaka University Medical School, 2-2 Yamadaoka, Suita, Osaka 565-0871, Japan

关键词: Splicing variant;    Orphan transporter;    Molecular cloning;    Cellular localization;    Bovine;   

DOI  :  10.1016/S0014-5793(98)01733-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have isolated a cDNA (bv7-3) encoding a member of the Na⁺,Cl⁻-dependent transporter family and its short splicing variant (bv7-3s) by screening a bovine retina cDNA library. Sequence analysis revealed that bv7-3 encodes a protein of 729 amino acids and is a bovine homologue of the rat orphan transporter v7-3-2. bv7-3s contains 265 amino acids, sharing 252 N-terminal amino acids with bv7-3. Both mRNAs for bv7-3 and bv7-3s were detected in nervous system by Northern blot analysis. In immunofluorescence analysis in transfected HEK 293T cells, myc-tagged bv7-3 was mainly detected on the plasma membrane, whereas myc-tagged bv7-3s showed a pattern of intracellular membrane staining.

【 授权许可】

Unknown