Characterization of a new family of proteins that interact with the C‐terminal region of the chromatin‐remodeling factor CHD‐31

FEBS Letters

Lemos, Taı́la A.¹  Nery, Flávia C.¹  Passos, Dario O.¹  Kobarg, Jörg¹ 

[1] Centro de Biologia Molecular Estrutural, Laboratório Nacional de Luz Sı́ncrotron, Rua Giuseppe Máximo Scolfaro 10.000, C.P. 6192, 13084-971 Campinas, SP, Brazil

关键词: Chromatin remodeling;    Protein–protein interaction;    Two-hybrid;    Domain mapping;    Cellular localization;    BV;    baculovirus;    CHD-3;    chromobox helicase DNA-binding domain protein-3;   

DOI  :  10.1016/S0014-5793(02)03737-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The two human proteins Ki-1/57 and CGI-55 have highly similar amino acid sequences but their functions are unknown. We analyzed them by yeast two-hybrid screens and found that they interact with the C-terminal region of the human chromatin-remodeling factor CHD-3 (chromo-helicase-DNA-binding domain protein-3). The interaction of CGI-55 and CHD-3 could be confirmed in vitro and in vivo by co-immunoprecipitations from Sf9 insect cells. Mapping showed that CGI-55 interacts with CHD-3 via two regions at its N- and C-terminals. The CGI-55 and Ki-1/57 mRNAs show highest expression in muscle, colon and kidney. A CGI55–GFP fusion protein was localized in the cytoplasm, nucleus and perinuclear regions of HeLa cells. These data suggest the possibility that CGI-55 and Ki-1/57 might be involved in nuclear functions like the remodeling of chromatin.

【 授权许可】

Unknown