Identification of a novel actin binding site within the Dp71 dystrophin isoform

FEBS Letters

Ray, Peter N.²  Howard, Perry L.²  Klamut, Henry J.¹ 

[1] Department of Experimental Therapeutics, Ontario Cancer Institute, Princess Margaret Hospital, and Department of Medical Biophysics, University of Toronto, 610 University Ave., Toronto, Ont. M5G 2M9, Canada;Department of Molecular and Medical Genetics, University of Toronto, Medical Sciences Building, Toronto, Ont. M5S 1A8, Canada

关键词: Dp71;    Dystrophin;    Actin binding site;    Duchenne muscular dystrophy;    Stress fiber;   

DOI  :  10.1016/S0014-5793(98)01566-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The Dp71 dystrophin isoform has recently been shown to localize to actin filament bundles in early myogenesis. We have identified an actin binding motif within Dp71 that is not found in other dystrophin isoforms. Actin overlay assays and transfection of COS-7 cells with fusion proteins of wild type and mutated Flag epitope-tagged Dp71 demonstrate that this motif is necessary and sufficient to direct localization of Dp71 to actin stress fibers. Furthermore, this localization is independent of alternative splicing which alters the C-terminus of the protein. The identification of an actin binding site suggests Dp71 may function to anchor membrane receptors to the cytoskeleton.

【 授权许可】

Unknown