Presence of long and short dystrophin and/or utrophin products in Torpedo marmorata peripheral nerves

FEBS Letters

Robert, Agnès¹  Mornet, Dominique¹  Rivier, François¹  Hugon, Gérald¹  Latouche, Jacqueline¹ 

[1] Pathologie Moléculaire du Muscle, INSERM U.300, Faculté de Pharmacie, Av. Charles Flahault, 34060 Montpellier cédex 1, France

关键词: Duchenne muscular dystrophy;    Dystrophin;    Dp116;    Up116;    Peripheral nerve;    Torpedo marmorata;    AChR;    acetylcholine receptor;    BMD;    Becker muscular dystrophy;    DAPC;    dystrophin associated protein complex;    DMD;    Duchenne muscular dystrophy;    Dps;    dystrophin products;    Dp71;    dystrophin product 71;    Dp116;    dystrophin product 116;    Dp140;    dystrophin product 140;    Dp260;    dystrophin product 260;    kDa;    kilodalton;    PAGE;    polyacrylamide gel electrophoresis;    SDS;    sodium dodecyl sulfate;    Up 116;    utrophin product 116;   

DOI  :  10.1016/0014-5793(95)01476-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Peripheral nerves from rabbit and Torpedo marmorata were comparatively analyzed for the presence of short dystrophin products. Western blot analyses of Torpedo marmorata peripheral nerve extracts revealed the existence of three proteins belonging to the dystrophin family: a M _r 400 kDa protein band detected with dystrophin/utrophin, dystrophin-specific and Torpedo utrophin-specific antibodies, a molecule identified as Dp116 and, for the first time at the protein level, a new protein probably corresponding to Up116. All of these products were carefully identified according to the specificities of the monoclonal antibodies used. In immunofluorescence studies, clear staining of the thin rim surrounding each Schwann cell-axon unit was observed in both Torpedo marmorata and rabbit peripheral nerves, showing colocalization of all of these molecules. Their potential functions were discussed in comparison to similar products found in rabbit peripheral nerves.

【 授权许可】

Unknown