Activation of p42/p44 mitogen‐activated protein kinases (MAPK) and p38 MAPK by tumor necrosis factor (TNF) is mediated through the death domain of the 55‐kDa TNF receptor

FEBS Letters

Boone, Elke¹  Vandevoorde, Veronique¹  Haegeman, Guy¹  De Wilde, Gert¹ 

[1] Department of Molecular Biology, University of Gent and Flanders Interuniversity Institute for Biotechnology, K.L. Ledeganckstraat 35, B-9000 Gent, Belgium

关键词: Death domain;    Mitogen-activated protein kinase;    Receptor;    Signaling;    Tumor necrosis factor;   

DOI  :  10.1016/S0014-5793(98)01567-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In the mouse fibrosarcoma cell line L929sA, tumor necrosis factor (TNF) stimulates activation of the stress-responsive p38 mitogen-activated protein kinase (MAPK), as well as the classical p42 and p44 MAPK. TNF signaling can be mediated by p55 or p75 TNF receptors. Here, we demonstrate that TNF-R55 is sufficient to activate p42/p44 MAPK and p38 MAPK. Moreover, by expressing different membrane-bound or purely cytoplasmic truncations of TNF-R55, we show that the intracellular death domain of TNF-R55 is the crucial domain involved. The cytoplasmic membrane-proximal region of TNF-R55, known to induce neutral sphingomyelinase activation, is not required for activation of p38 MAPK or p42/p44 MAPK.

【 授权许可】

Unknown