A protein related to a proteasomal subunit binds to the intracellular domain of the p55 TNF receptor upstream to its ‘death domain’

FEBS Letters

Wallach, David¹  Boldin, Mark P.¹  Mett, Igor L.¹ 

[1] Department of Membrane Research and Biophysics, The Weizmann Institute of Science, Rehovot, 76100, Israel

关键词: Death domain;    Receptor;    Proteasome;    Signaling;    Tumor necrosis factor;    Two-hybrid system;    GST;    glutathione S-transferase;    Fas-IC;    intracellular domain of Fas/APO1;    FLAG-55.11;    the region extending between residues 309 and 900 in the 55.11 protein and which was N-linked to the FLAG octapeptide;    IC;    intracellular domain;    p55-R;    the p55 receptor for TNF;    p75-R;    the p75 receptor for TNF;    p55-IC;    intracellular domain of the p55-R;    TNF;    tumor necrosis factor;   

DOI  :  10.1016/0014-5793(95)00534-G

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A novel protein that binds specifically to the intracellular domain of the p55 tumor necrosis factor (TNF) receptor was cloned by two-hybrid screening of a HeLa cell cDNA library. Data bank searches revealed high sequence similarity of the protein (55.11) to yeast, nematode and plant proteins, whose functions are yet unknown. Significant similarity was also found between 55.11 and SEN3, the yeast equivalent of the p112 subunit of the 26S proteasome. Deletion analysis showed that the protein binds to the p55 receptor upstream to the region involved in induction of cell death.

【 授权许可】

Unknown