Proteasomal activity modulates TGF‐ß signaling in a gene‐specific manner

FEBS Letters

Mönkkönen, Mia¹  Roth, Stina¹  Laiho, Marikki¹  Zhang, Fan¹ 

[1] Haartman Institute, Department of Virology and Molecular Cancer Biology Program, Biomedicum Helsinki, University of Helsinki and Helsinki University Central Hospital Laboratory Diagnostics, P.O. Box 63, FIN-00014 Helsinki, Finland

关键词: Transforming growth factor-β;    Smad;    SnoN;    Proteasome;    Signaling;   

DOI  :  10.1016/S0014-5793(02)03163-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Transforming growth factor-β (TGF-β) signaling relies on Smad-signaling pathway controlled in part by the proteasome. Here we demonstrate that inhibition of the proteasome function in mink epithelial cells accumulates both positive and negative modulators of TGF-β signaling, phospho-Smad2 and SnoN. Inhibition of the proteasome led to abrogation of TGF-β target gene regulation in a gene-specific manner. While regulation of p15Ink4b and myc by TGF-β are lost, PAI-1 induction, previously shown to occur in a Smad3-dependent manner, was not affected by treatment of the cells with the proteasomal inhibitor MG132. The results suggest that proteasomal activity is required for TGF-β signaling in a gene-specific manner.

【 授权许可】

Unknown