Antibodies against type II phospholipase A2 prevent renal injury due to ischemia and reperfusion in rats

FEBS Letters

Tanaka, Hideyuki¹  Kawauchi, Yasushi¹  Takasaki, Jun¹  Usuda, Shinji¹  Masuho, Yasuhiko¹  Urasaki, Tetsuya¹ 

[1] Institute for Drug Discovery Research, Yamanouchi Pharmaceutical Co. Ltd., 21 Miyukigaoka, Tsukuba, Ibaraki, 305-8585, Japan

关键词: Phospholipase A2;    Ischemia;    Myeloperoxidase;    Renal failure;    PLA2-II;    type II phospholipase A2;    MPO;    myeloperoxidase;    BUN;    blood urea nitrogen;   

DOI  :  10.1016/S0014-5793(98)01490-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

This study was performed to determine the involvement of type II phospholipase A₂ (PLA₂-II) in renal injury caused by ischemia and reperfusion. Ischemia and reperfusion significantly elevated levels of blood urea nitrogen and serum creatinine in rats. These increases were significantly reduced by i.v. administration of rabbit IgG F(ab′)2 fragments against rat PLA₂-II. Increased levels of acid-stable PLA₂ activity in the kidney were caused by ischemia and reperfusion, and were suppressed by administration of anti-PLA₂-II F(ab′)2. Increased levels of myeloperoxidase activity, a marker of neutrophil infiltration, in the kidney were also reduced after anti-PLA₂-II F(ab′)2 treatment. These results suggest that PLA₂-II plays a pivotal role in pathogenesis of ischemia and reperfusion injury through induction of neutrophil infiltration.

【 授权许可】

Unknown