Preconditioning somatothermal stimulation on Qimen (LR14) reduces hepatic ischemia/reperfusion injury in rats

BMC Complementary and Alternative Medicine

Ying-Ling Wu⁴  Jen-Hwey Chiu¹  Shu-Chen Hsieh²  Cheng-Chu Hsieh³ 

[1]Institute of Traditional Medicine, School of Medicine, National Yang-Ming University, Taipei 112, Taiwan

[2]Institute of Food Science and Technology, National Taiwan University, Taipei 106, Taiwan

[3]Biologics Division, Animal Health Research Institute, Council of Agriculture, Executive Yuan, New Taipei City 251, Taiwan

[4]Department and Institute of Veterinary Medicine, School of Veterinary Medicine, National Taiwan University, Taipei 106, Taiwan

关键词: Myeloperoxidase;    Superoxidase dismutase;    Qimen (LR14);    Ischemia-reperfusion;   

Others  :  1220343
DOI  :  10.1186/1472-6882-14-18

 received in 2013-06-21, accepted in 2014-01-06,  发布年份 2014

【 摘 要 】

Background

In human beings or animals, ischemia/reperfusion (I/R) injury of the liver may occur in many clinical conditions, such as circulating shock, liver transplantation and surgery and several other pathological conditions. I/R injury has a complex pathophysiology resulting from a number of contributing factors. Therefore, it is difficult to achieve effective treatment or protection by individually targeting the mediators. This study aimed at studying the effects of local somatothermal stimulation preconditioning on the right Qimen (LR14) on hepatic I/R injury in rats.

Methods

Eighteen male Sprague-Dawley rats were randomly divided into three groups. The rats were preconditioned with thermal tolerance study, which included one dose of local somatothermal stimulation (LSTS) on right Qimen (LR14) at an interval of 12 h, followed by hepatic ischemia for 60 min and then reperfusion for 60 min. Serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT) have been used to assess the liver functions, and liver tissues were taken for the measurements such as malondialdehyde (MDA), glutathione (GSH), catalase (CAT), superoxidase dismutase (SOD), and myeloperoxidase (MPO).

Results

The results show that the plasma ALT and AST activities were higher in the I/R group than in the control group. In addition, the plasma ALT and AST activities decreased in the groups that received LSTS. The hepatic SOD levels reduced significantly by I/R injury. Moreover, the hepatic MPO activity significantly increased by I/R injury while it decreased in the groups given LSTS.

Conclusions

Our findings show that LSTS provides a protective effects on the liver from the I/R injury. Therefore, LSTS might offer an easy and inexpensive intervention for patients who have suffered from I/R of the liver especially in the process of hepatotomy and hepatic transplantation.

【 授权许可】

   
2014 Hsieh et al.; licensee BioMed Central Ltd.

【 图 表 】

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【 参考文献 】

• [1]de la Monte SM, Arcidi JM, Moore GW, Hutchins GM: Midzonal necrosis as a pattern of hepatocellular injury after shock. Gastroenterology 1984, 86(4):627-631.
• [2]Yoshikawa T, Murakami M, Yoshida N, Seto O, Kondo M: Effects of superoxide dismutase and catalase on disseminated intravascular coagulation in rats. Thromb Haemost 1983, 50(4):869-872.
• [3]Arthur MJ: Reactive oxygen intermediates and liver injury. J Hepatol 1988, 6(1):125-131.
• [4]Jungermann K, Kietzmann T: Oxygen: modulator of metabolic zonation and disease of the liver. Hepatology 2000, 31(2):255-260.
• [5]Lemasters JJ, Ji S, Thurman RG: Centrilobular injury following hypoxia in isolated, perfused rat liver. Science 1981, 213(4508):661-663.
• [6]Gonzalez-Flecha B, Cutrin JC, Boveris A: Time course and mechanism of oxidative stress and tissue damage in rat liver subjected to in vivo ischemia-reperfusion. J Clin Invest 1993, 91(2):456-464.
• [7]Halliwell B: Reactive oxygen species in living systems: source, biochemistry, and role in human disease. Am J Med 1991, 91(3C):14S-22S.
• [8]McCord JM: Oxygen-derived free radicals in postischemic tissue injury. N Engl J Med 1985, 312(3):159-163.
• [9]Vollmar B, Glasz J, Leiderer R, Post S, Menger MD: Hepatic microcirculatory perfusion failure is a determinant of liver dysfunction in warm ischemia-reperfusion. Am J Pathol 1994, 145(6):1421-1431.
• [10]Jaattela M, Wissing D, Kokholm K, Kallunki T, Egeblad M: Hsp70 exerts its anti-apoptotic function downstream of caspase-3-like proteases. EMBO J 1998, 17(21):6124-6134.
• [11]Inoue M, Kitakoji H, Yano T, Ishizaki N, Itoi M, Katsumi Y: Acupuncture treatment for low back pain and lower limb symptoms-the relation between acupuncture or electroacupuncture stimulation and sciatic nerve blood flow. Evid Based Complement Alternat Med 2008, 5(2):133-143.
• [12]Hua JS, Li LP, Zhu XM: Effects of moxibustion pretreating on SOD and MDA in the rat of global brain ischemia. J Tradit Chin Med 2008, 28(4):289-292.
• [13]Pang Y, Wu LB, Liu DH: Acupuncture therapy for apoplectic aphasia: a systematic review. Zhongguo Zhen Jiu 2010, 30(7):612-616.
• [14]Berman BM, Langevin HM, Witt CM, Dubner R: Acupuncture for chronic low back pain. N Engl J Med 2010, 363(5):454-461.
• [15]Lee MS, Choi TY, Kang JW, Lee BJ, Ernst E: Moxibustion for treating pain: a systematic review. Am J Chin Med 2010, 38(5):829-838.
• [16]Beyerman KL, Palmerino MB, Zohn LE, Kane GM, Foster KA: Efficacy of treating low back pain and dysfunction secondary to osteoarthritis: chiropractic care compared with moist heat alone. J Manipulative Physiol Ther 2006, 29(2):107-114.
• [17]Yeh CC, Ko SC, Huh BK, Kuo CP, Wu CT, Cherng CH, Wong CS: Shoulder tip pain after laparoscopic surgery analgesia by collateral meridian acupressure (shiatsu) therapy: a report of 2 cases. J Manipulative Physiol Ther 2008, 31(6):484-488.
• [18]Arroyo-Morales M, Olea N, Martinez M, Moreno-Lorenzo C, Diaz-Rodriguez L, Hidalgo-Lozano A: Effects of myofascial release after high-intensity exercise: a randomized clinical trial. J Manipulative Physiol Ther 2008, 31(3):217-223.
• [19]Pfefer MT, Cooper SR, Uhl NL: Chiropractic management of tendinopathy: a literature synthesis. J Manipulative Physiol Ther 2009, 32(1):41-52.
• [20]Pan PJ, Chan RC, Yang AH, Chou CL, Cheng YF, Chiu JH: Protective effects of preconditioned local somatothermal stimulation on neuromuscular plasticity against ischemia–reperfusion injury in rats. J Orthop Res 2008, 26(12):1670-1674.
• [21]Lin YH, Chiu JH, Tung HH, Tsou MT, Lui WY, Wu CW: Preconditioning somatothermal stimulation on right seventh intercostal nerve territory increases hepatic heat shock protein 70 and protects the liver from ischemia-reperfusion injury in rats. J Surg Res 2001, 99(2):328-334.
• [22]Chiu JH, Lui WY, Chen YL, Hong CY: Local somatothermal stimulation inhibits the motility of sphincter of Oddi in cats, rabbits and humans through nitrergic neural release of nitric oxide. Life Sci 1998, 63(6):413-428.
• [23]Chiu JH, Tsou MT, Tung HH, Tai CH, Tsai SK, Chih CL, Lin JG, Wu CW: Preconditioned somatothermal stimulation on median nerve territory increases myocardial heat shock protein 70 and protects rat hearts against ischemia-reperfusion injury. J Thorac Cardiovasc Surg 2003, 125(3):678-685.
• [24]Jiang JK, Chiu JH, Lin JK: Local somatothermal stimulation inhibits motility of the internal anal sphincter through nitrergic neural release of nitric oxide. Dis Colon Rectum 2000, 43(3):381-388.
• [25]Hiratsuka M, Yano M, Mora BN, Nagahiro I, Cooper JD, Patterson GA: Heat shock pretreatment protects pulmonary isografts from subsequent ischemia-reperfusion injury. J Heart Lung Transplant 1998, 17(12):1238-1246.
• [26]Canada AT, Stein K, Martel D, Watkins WD: Biochemical appraisal of models for hepatic ischemia-reperfusion injury. Circ Shock 1992, 36(3):163-168.
• [27]Chiu JH, Ho CT, Wei YH, Lui WY, Hong CY: In vitro and in vivo protective effect of honokiol on rat liver from peroxidative injury. Life Sci 1997, 61(19):1961-1971.
• [28]Hillegass LM, Griswold DE, Brickson B, Albrightson-Winslow C: Assessment of myeloperoxidase activity in whole rat kidney. J Pharmacol Methods 1990, 24(4):285-295.
• [29]Koo A, Komatsu H, Tao G, Inoue M, Guth PH, Kaplowitz N: Contribution of no-reflow phenomenon to hepatic injury after ischemia-reperfusion: evidence for a role for superoxide anion. Hepatology 1992, 15(3):507-514.
• [30]Deshaies RJ, Koch BD, Werner-Washburne M, Craig EA, Schekman R: A subfamily of stress proteins facilitates translocation of secretory and mitochondrial precursor polypeptides. Nature 1988, 332(6167):800-805.
• [31]Wienhues U, Neupert W: Protein translocation across mitochondrial membranes. Bioessays 1992, 14(1):17-23.
• [32]Jaeschke H: Molecular mechanisms of hepatic ischemia-reperfusion injury and preconditioning. Am J Physiol Gastrointest Liver Physiol 2003, 284(1):G15-G26.
• [33]Liu Z, Xu Z, Shen W, Li Y, Zhang J, Ye X: Effect of pharmacologic preconditioning with tetrandrine on subsequent ischemia/reperfusion injury in rat liver. World J Surg 2004, 28(6):620-624.
• [34]Oreopoulos GD, Wu H, Szaszi K, Fan J, Marshall JC, Khadaroo RG, He R, Kapus A, Rotstein OD: Hypertonic preconditioning prevents hepatocellular injury following ischemia/reperfusion in mice: a role for interleukin 10. Hepatology 2004, 40(1):211-220.
• [35]Takahashi Y, Tamaki T, Tanaka M, Konoeda Y, Kawamura A, Katori M, Kakita A: Efficacy of heat-shock proteins induced by severe fasting to protect rat livers preserved for 72 hours from cold ischemia/reperfusion injury. Transplant Proc 1998, 30(7):3700-3702.
• [36]Vajdova K, Heinrich S, Tian Y, Graf R, Clavien PA: Ischemic preconditioning and intermittent clamping improve murine hepatic microcirculation and Kupffer cell function after ischemic injury. Liver Transpl 2004, 10(4):520-528.
• [37]Latchman DS: Heat shock proteins and cardiac protection. Cardiovasc Res 2001, 51(4):637-646.
• [38]Parsell DA, Lindquist S: The function of heat-shock proteins in stress tolerance: degradation and reactivation of damaged proteins. Annu Rev Genet 1993, 27:437-496.
• [39]Russell WJ, Jackson RM: MnSOD protein content changes in hypoxic/hypoperfused lung tissue. Am J Respir Cell Mol Biol 1993, 9(6):610-616.
• [40]Knowlton AA, Kapadia S, Torre-Amione G, Durand JB, Bies R, Young J, Mann DL: Differential expression of heat shock proteins in normal and failing human hearts. J Mol Cell Cardiol 1998, 30(4):811-818.
• [41]Weisiger RA, Fridovich I: Superoxide dismutase, organelle specificity. J Biol Chem 1973, 248(10):3582-3592.
• [42]Javadpour M, Kelly CJ, Chen G, Bouchier-Hayes DJ: Herbimycin-A attenuates ischaemia-reperfusion induced pulmonary neutrophil infiltration. Eur J Vasc Endovasc Surg 1998, 16(5):377-382.
• [43]Javadpour M, Kelly CJ, Chen G, Stokes K, Leahy A, Bouchier-Hayes DJ: Thermotolerance induces heat shock protein 72 expression and protects against ischaemia-reperfusion-induced lung injury. Br J Surg 1998, 85(7):943-946.
• [44]Chen G, Kelly C, Stokes K, Wang JH, Leahy A, Bouchier-Hayes D: Induction of heat shock protein 72 kDa expression is associated with attenuation of ischaemia-reperfusion induced microvascular injury. J Surg Res 1997, 69(2):435-439.
• [45]Stojadinovic A, Kiang J, Smallridge R, Galloway R, Shea-Donohue T: Induction of heat-shock protein 72 protects against ischemia/reperfusion in rat small intestine. Gastroenterology 1995, 109(2):505-515.
• [46]Weiss SJ, Ward PA: Immune complex induced generation of oxygen metabolites by human neutrophils. Journal of immunology 1982, 129(1):309-313.
• [47]Heinzelmann M, Mercer-Jones MA, Passmore JC: Neutrophils and renal failure. Am J Kidney Dis 1999, 34(2):384-399.
• [48]Zimmerman BJ, Grisham MB, Granger DN: Role of oxidants in ischemia/reperfusion-induced granulocyte infiltration. Am J Physiol 1990, 258(2 Pt 1):G185-G190.