Expression of a functional barley sucrose‐fructan 6‐fructosyltransferase in the methylotrophic yeast Pichia pastoris

FEBS Letters

De Virgilio, Claudio¹  Lüscher, Marcel¹  Hochstrasser, Urs¹  Wiemken, Andres¹  Boller, Thomas¹ 

[1] Department of Botany, University of Basel, Hebelstrasse 1, CH-4056 Basel, Switzerland

关键词: Fructan;    Heterologous expression;    Sucrose-fructan 6-fructosyltransferase;    Hordeum vulgare;    Pichia pastoris;    6-SFT;    sucrose-fructan 6-fructosyltransferase;    1-SST;    sucrose-sucrose 1-fructosyltransferase;    6-SST;    sucrose-sucrose 6-fructosyltransferase;    AOX1;    alcohol oxidase 1;    BMGY;    buffered complex glycerol medium;    BMMY buffered complex methanol medium;    DP;    degree of polymerization;    MES;    2-morpholinoethanesulfonic acid;    ORF;    open reading frame;    PCR;    polymerase chain reaction;    YPDS;    rich;    complex broth medium with dextrose and sorbitol;   

DOI  :  10.1016/S0014-5793(98)01487-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The cDNA encoding sucrose-fructan 6-fructosyltransferase (6-SFT) from barley (Hordeum vulgare) has been expressed in the methylotrophic yeast Pichia pastoris, using a translational fusion into vector pPICZαC, containing the N-terminal signal sequence of Saccharomyces cerevisiae α-factor to allow entry into the secretory pathway. Transformed Pichia produced and secreted a functional 6-SFT which had characteristics similar to the barley enzyme, but had a pronounced additional 1-SST activity when incubated with sucrose.

【 授权许可】

Unknown