Role of CFTR and anion exchanger in bicarbonate fluxes in C127 cell lines

FEBS Letters

Rugolo, Michela¹  Mastrocola, Teresa¹  Porcelli, Anna Maria¹ 

[1] Dipartimento di Biologia Ev.Sp., Università di Bologna, Via Irnerio 42, 40126 Bologna, Italy

关键词: Intracellular pH;    HCO− 3 transport;    Cystic fibrosis;    Cystic fibrosis transmembrane conductance regulator;    Anion exchanger;    CF;    cystic fibrosis;    CFTR;    cystic fibrosis transmembrane conductance regulator;    ΔF508;    deletion of Phe508;    wt;    wild type;    IBMX;    3-isobutyl-1-methylxanthine;    BCECF-AM;    2′;    7′-bis(2-carboxyethyl)-5(6)-carboxyfluorescein tetraacetoxymethyl ester;    pHi;    intracellular pH;    H2DIDS;    4;    4′-diisothiocyanate dihydrostilbene-2;    2′-disulfonic acid;   

DOI  :  10.1016/S0014-5793(98)01468-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

C127 cell lines transfected with wtCFTR, ΔF508CFTR or vector were employed to determine HCO⁻ ₃ fluxes in the presence or absence of functional CFTR, using the pH-sensitive dye BCECF. Both cytosolic alkalinization and acidification were due to activity of anion exchanger and were similar in the three cell lines, indicating that expression of CFTR did not influence anion exchanger activity. In C127wt cells only, cAMP elevating agents significantly stimulated HCO⁻ ₃ fluxes, insensitive to the inhibitor of anion exchanger 4,4′-diisothiocyanate dihydrostilbene-2,2′-disulfonic acid, suggesting that activated CFTR directly mediates both HCO⁻ ₃ influx and efflux and therefore can contribute to intracellular and extracellular pH regulation.

【 授权许可】

Unknown