The vacuolating toxin of Helicobacter pylori mimicks the CFTR‐mediated chloride conductance1

FEBS Letters

Cabrini, Giulio²  Tombola, Francesco¹  Zoratti, Mario¹  Campello, Silvia¹ 

[1] CNR Institute of Neuroscience, Section for Biomembranes, and Department of Biomedical Sciences, University of Padova, Viale G. Colombo 3, 35121 Padua, Italy;Laboratory of Molecular Pathology, Cystic Fibrosis Center, Piazzale Stefani 1, 37126 Verona, Italy

关键词: VacA;    Cystic fibrosis;    Cystic fibrosis transmembrane conductance regulator;    Chloride channel;    Respiratory epithelium;    Helicobacter pylori;    ASL;    airway surface liquid;    BEGM;    bronchial epithelial growth medium;    CF;    cystic fibrosis;    CFTR;    cystic fibrosis transmembrane conductance regulator;    DMEM;    Dulbecco's minimum Eagle's medium;    FCS;    fetal calf serum;    NPPB;    5-nitro-2-(3-phenylpropylamino) benzoic acid;   

DOI  :  10.1016/S0014-5793(02)03656-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Cystic fibrosis (CF) is caused by defects of the CF transmembrane conductance regulator (CFTR), which acts both as an anion-selective channel and as a regulator of other proteins. The relative contribution of these two functions in CF disease is debated. The toxin VacA forms channels with properties similar to those of the CFTR, and we report here that it can insert into the membrane of various cells originating from respiratory epithelia, generating a chloride conductance comparable to that produced by activation of the CFTR. VacA may therefore become a valuable tool in the study of CF pathogenesis.

【 授权许可】

Unknown