Translocation of acylated pardaxin into cells

FEBS Letters

Adermann, Knut³  Erdmann, Georg²  Lazarovici, Philip¹  Paul, Yasmin²  Hochman, Jacob⁴  Weiss, Aryeh⁵  Wellhöner, Hans²  Kassebaum, Carola² 

[1] Department of Pharmacology and Experimental Therapeutics, School of Pharmacy, Faculty of Medicine, Hebrew University of Jerusalem, Jerusalem 91120, Israel;Institute of Toxicology, Medical School of Hannover, Carl-Neuberg-Str., D-30623 Hannover, Germany;Lower Saxony Institute of Peptide Research, Feodor-Lynen-Str. 31, D-30625 Hannover, Germany;Department of Cell and Animal Biology, Institute of Life Sciences, Hebrew University of Jerusalem, Jerusalem 91904, Israel;Department of Biochemistry, Institute of Life Sciences, Hebrew University of Jerusalem, Jerusalem 91904, Israel

关键词: Pardaxin uptake;    Peptide uptake;    BCECF-AM;    2′;    7′-bis-(2-carboxyethyl)-5- (and -6-)carboxyfluorescein acetoxymethylester;    Bolton-Hunter reagent;    succinimidyl-3-(3-125I-4-hydroxy)phenylpropionate;    HPLC;    high performance liquid chromatography;    HPP;    4-hydroxyphenylpropionyl;    ITH;    iminothiolane;    MCC;    4-(N-maleimidomethyl)cyclohexane-1-carboxyl;    PDP;    6-[3′-(2-pyridyldithio)propionamido]hexanoyl;    PYR;    pyrenyldiazomethane;    SHPP (Rudinger's reagent);    sulfosuccinimidyl-3-(4-hydroxy)phenylpropionate;    slcSPDP;    sulfosuccinimidyl-6-[3′-(2-pyridyldithio)propionamido]hexanoate;    sSMCC;    sulfosuccinimidyl-4-(N-maleimidomethyl)cyclohexane-1-carboxylate;   

DOI  :  10.1016/S0014-5793(98)01389-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Acylated pardaxin is translocated through the cytoplasmic membrane and is accumulated in the nucleoli of NG108-15 and chromaffin cells. The uptake is time- and dose-dependent and temperature-sensitive. However, the binding of acylated ¹²⁵I-pardaxin cannot be reduced by competition with pardaxin acylated with Rudinger's reagent. In this respect, acylated pardaxin resembles the Tat protein 37–71 fragment. Metabolic inhibitors do not significantly reduce the uptake of acylated ¹²⁵I-pardaxin. Acylated pardaxin might be useful as a vector to translocate other molecules.

【 授权许可】

Unknown