Glyoxylate cycle enzymes are present in liver peroxisomes of alloxan‐treated rats

FEBS Letters

Eprintsev, Alexander T¹  Igamberdiev, Abir U¹  Volvenkin, Sergei V¹  Popov, Vasily N¹ 

[1] Department of Plant Physiology and Biochemistry, Voronezh State University, Voronezh 394693, Russia

关键词: Glyoxylate cycle;    Isocitrate lyase;    Malate synthase;    Induction;    Alloxan;    Diabetes;    AH;    aconitate hydratase;    CS;    citrate synthase;    GC;    glyoxylate cycle;    ICL;    isocitrate lyase;    MDH;    malate dehydrogenase;    MS;    malate synthase;    PPAR;    peroxisome proliferator-activated receptor;   

DOI  :  10.1016/S0014-5793(98)01422-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Key enzymes of the glyoxylate cycle, isocitrate lyase (ICL) and malate synthase (MS), have been detected in the liver of alloxan-treated rats. The activity of ICL in rat liver was 0.040 μmol/min/mg protein and the activity of MS was 0.022 μmol/min/mg protein. These enzymes were associated with the peroxisomal fraction. The activities of citrate synthase, malate synthase and malate dehydrogenase detected in the peroxisomal fraction were also increased by alloxan treatment. Isocitrate lyase was partially purified and displayed catalytic and regulatory properties similar to those of the enzyme isolated from the liver of starved rats (Popov, V.N. et al. (1996) FEBS Lett. 391, 87–90).

【 授权许可】

Unknown