Operation of glyoxylate cycle in halophilic archaea: presence of malate synthase and isocitrate lyase in Haloferax volcanii

FEBS Letters

Camacho, Mónica¹  Bonete, Maria José¹  Serrano, Juan Antonio¹ 

[1] División de Bioquı́mica, Facultad de Ciencias, Universidad de Alicante, Ap. 99, 03080 Alicante, Spain

关键词: Glyoxylate cycle;    Malate synthase;    Isocitrate lyase;    Isocitrate dehydrogenase;    Archaeon;    Haloferax volcanii;   

DOI  :  10.1016/S0014-5793(98)00911-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The occurrence of the glyoxylate cycle has not previously been demonstrated in any of the Archaea. In halophilic archaea, only isocitrate lyase activity has been detected. The halophilic archaeon Haloferax volcanii was tested for the presence of the other key enzyme of this pathway, malate synthase. High activities of this enzyme were detected when the carbon source was acetate. Both glyoxylate cycle key enzymes, isocitrate lyase and malate synthase, from Hf. volcanii were purified and characterized.

【 授权许可】

Unknown