Transformed but not normal hepatocytes express UCP2

FEBS Letters

Torres, Luis¹  Mato, José M¹  Carretero, M.Victoria¹  Avila, Matı́as A¹  Latasa, M.Ujue¹  Garcı́a-Trevijano, Elena R¹  Prieto, Jesús¹ 

[1] División de Hepatologı́a y Terapia Génica, Departamento de Medicina Interna, Facultad de Medicina, Universidad de Navarra, 31080 Pamplona, Spain

关键词: Uncoupling protein;    Hepatoma;    Mitochondrion;    Tumor marker;    Hypomethylation;    UCP2;    uncoupling protein 2;    DD;    differential display;    HCC;    hepatocellular carcinoma;    PMA;    phorbol 12-myristate 13-acetate;    PKC;    protein kinase C;    AFP;    α-fetoprotein;   

DOI  :  10.1016/S0014-5793(98)01335-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Uncoupling protein 2 (UCP2) expression in liver is restricted to non-parenchymal cells. By means of differential display screening between normal rat liver and H4IIE hepatoma cells we have isolated a cDNA clone encompassing part of UCP2 cDNA. Northern blot analysis revealed that UCP2 is expressed in some hepatocarcinoma cell lines, while it is absent in adult hepatocytes. UCP2 mRNA in H4IIE cells was downregulated when cells were cultured for 36 h in 0.1% serum and its expression was restored upon addition of 10% serum or phorbol esters. Hypomethylation of UCP2 was observed in transformed UCP2 expressing cells. Our results indicate that UCP2 is expressed in some hepatocarcinoma cell lines and that serum components may participate in maintaining elevated UCP2 levels.

【 授权许可】

Unknown