FEBS Letters | |
Genomic organisation, alternative splicing and primary structure of human matrilin‐4 | |
Wagener, Raimund1  Paulsson, Mats1  Kobbe, Birgit1  | |
[1] Institute for Biochemistry II, Medical Faculty, University of Cologne, Joseph-Stelzmann-Str. 52, D-50931 Cologne, Germany | |
关键词: Matrilin; Extracellular matrix; Von Willebrand factor type A; Epidermal growth factor; Coiled-coil α-helix; AT-AC intron; vWFA; von Willebrand factor type A; EGF; epidermal growth factor; EST; expressed sequence tag; RT-PCR; reverse transcription polymerase chain reaction; HTGS; high-throughput genomic sequence; | |
DOI : 10.1016/S0014-5793(98)01293-9 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
We have recently cloned a cDNA for mouse matrilin-4. By sequence comparison we identified the 12 kb long human matrilin-4 gene as a part of a high-throughput genomic sequence (HS453C12) in the databases. Additionally we found a human matrilin-4 expressed sequence tag (H54037) in the database that had been mapped to chromosome 20q13.1–2. The gene contains 10 exons and, like the matrilin-1 gene, the human matrilin-4 gene contains an AT-AC intron between the two exons en coding the coiled-coil domain. The cDNA sequence of human matrilin-4 was determined by sequencing of RT-PCR products obtained from mRNA of the human embryonic kidney cell line HEK 293. At the amino acid level it showed an overall sequence identity to the mature mouse matrilin-4 of 91% with a maximum of 97% in the second vWFA-like module. Alternative splicing leads to three different mRNAs. They all encode the putative signal peptide, the two vWFA-like domains and the potential coiled-coil α-helical oligomerisation domain but differ in that either one, two or three EGF-like domains are retained in the mature mRNA. Due to a G to A mutation at the splice donor site of intron C, the third exon encodes an untranslated pseudo-exon specifying the first EGF-like domain when compared to mouse matrilin-4.
【 授权许可】
Unknown
【 预 览 】
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RO201912020306761ZK.pdf | 174KB | download |