Matrilin‐4, a new member of the matrilin family of extracellular matrix proteins

FEBS Letters

Wagener, Raimund¹  Paulsson, Mats¹  Kobbe, Birgit¹ 

[1] Institute for Biochemistry II, Medical Faculty, University of Cologne, Joseph-Stelzmann-Str. 52, D-50931 Cologne, Germany

关键词: Matrilin;    Extracellular matrix;    Von Willebrand factor type A;    Epidermal growth factor;    Coiled-coil α-helix;    Alternative splicing;    vWFA;    von Willebrand factor type A;    EGF;    epidermal growth factor;    EST;    expressed sequence tag;    GAPDH;    glyceraldehyde-3-phosphate dehydrogenase;    RT-PCR;    reverse transcription polymerase chain reaction;    RACE;    rapid amplification of cDNA ends;   

DOI  :  10.1016/S0014-5793(98)01111-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Mouse cDNA encoding for matrilin-4 was cloned and the primary structure of this fourth member of the matrilin family was deduced from the nucleotide sequence. The protein precursor of 624 amino acids consists of a putative signal peptide, two vWFA-like domains linked by four epidermal growth factor-like modules and a potential coiled-coil α-helical oligomerization domain at the C-terminus. The predicted M _r of the mature protein is 66 442. Expression in lung, brain, sternum, kidney and heart was detected by Northern blot analysis of mouse mRNA. Additionally an alternatively spliced mRNA lacking the sequence coding for the first vWFA domain was found in 7 weeks old mice leading to a protein precursor of 434 amino acids and a predicted M _r of the mature protein of 45 468.

【 授权许可】

Unknown