Matrilin‐3 from chicken cartilage

FEBS Letters

Belluoccio, Daniele¹  Trueb, Beat¹ 

[1] MEM Institute, Division of Biology, University of Bern, P.O. Box 30, CH-3010 Bern, Switzerland

关键词: Cartilage;    Epidermal growth factor;    Extracellular matrix;    Matrilin;    Subtractive cDNA cloning;    Von Willebrand factor;    CMP;    cartilage matrix protein (matrilin-1);    EGF domain;    epidermal growth factor-like domain;    EST;    expressed sequence tag;    PCR;    polymerase chain reaction;    vWFA domain;    von Willebrand factor A-like domain;   

DOI  :  10.1016/S0014-5793(97)01126-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

By subtractive cDNA cloning we have identified a novel constituent of chicken cartilage termed matrilin-3. This constituent is encoded by a mRNA of 2.2 kbp whose expression is restricted to cartilaginous tissues. The predicted protein is composed of 452 amino acids with a molecular mass of 49 kDa. It contains a single von Willebrand factor A-like domain, four epidermal growth factor-like repeats and an α-helical region which may induce the formation of oligomers via a coiled-coil. The primary structure is similar to that of matrilin-1 which is also expressed in a cartilage-specific manner. This similarity suggests that the genes for the two proteins may have evolved from a common ancestor by gene duplication.

【 授权许可】

Unknown