Growth factor protection against cytokine‐induced apoptosis in neonatal rat islets of Langerhans: role of Fas

FEBS Letters

Harrison, M.²  Green, I.C.²  Green, M.H.L.³  Dunger, A.M.¹  John, N.²  Mabley, J.²  Berg, S.¹ 

[1] Institut für Diabetes ‘Gerhardt Katsch’, Karlsburg, Germany;Department of Biochemistry, School of Biological Sciences, University of Sussex, Falmer, Brighton BN1 9QG, E. Sussex, UK;MRC Cell Mutation Unit, University of Sussex, Brighton BN1 9RR, UK

关键词: Growth factor;    Cytokine;    Fas;    Apoptosis;    Islet of Langerhans;   

DOI  :  10.1016/S0014-5793(98)01051-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Treatment of neonatal rat islets of Langerhans with combined cytokines (interleukin-1β 10⁻¹⁰ M, tumour necrosis factor-α 10⁻¹⁰ M, interferon-γ 5 U/ml) led to extensive cell death, which was potentiated by Fas activation with the anti-Fas cytolytic antibody JO2. Pre-treatment with insulin (25 ng/ml) or insulin-like growth factor-1 (10⁻⁸ M) gave only partial protection against cell killing, but prevented the Fas-mediated component. In the absence of cytokine treatment, Fas-mediated killing was not observed.

【 授权许可】

Unknown