Density‐ and proliferation status‐dependent expression of T‐cadherin, a novel lipoprotein‐binding glycoprotein: a function in negative regulation of smooth muscle cell growth?

FEBS Letters

Kern, Frances²  Resink, Thérèse J²  Tkachuk, Vsevolod A¹  Kuzmenko, Yelena S²  Bochkov, Valery N¹ 

[1] Laboratory for Molecular Endocrinology, Cardiology Research Center, Moscow, Russia;Department of Research, Laboratory for Cardiovascular Research, Basel University Hospital, CH 4031 Basel, Switzerland

关键词: T-cadherin;    Low density lipoprotein;    Smooth muscle cell;    LDL;    low density lipoprotein;    SMC;    vascular smooth muscle cell;    LPDS;    lipoprotein-deficient serum;    FCS;    fetal calf serum;    SF;    serum-free;    PDGF;    platelet-derived growth factor;    EGF;    epidermal growth factor;    IGF;    insulin-like growth factor;    TBS;    Tris-buffered saline;    HRP;    horseradish peroxidase;    ECL;    enhanced chemiluminescence;   

DOI  :  10.1016/S0014-5793(98)00977-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The atypical low density lipoprotein (LDL) binding proteins (M _r 105 and 130 kDa; p105 and p130) in human aortic medial membranes and cultured human and rat aortic smooth muscle cells (SMC) have recently been identified as the cell adhesion glycoprotein T-cadherin. Although cadherins are generally recognized to be important regulators of morphogenesis, the function of T-cadherin in the vasculature is poorly understood. This study has examined the relationship between expression of T-cadherin and the density and proliferation status of SMC. T-cadherin (p105 and p130) levels in SMC lysates were measured on Western blots using ligand-binding techniques. T-cadherin expression was dependent upon cell density, and maximal levels were achieved at confluency. T-cadherin levels were reversibly modulated by switching cultures between serum-free (upmodulation) and serum-containing (downmodulation) conditions. Platelet-derived growth factor (PDGF)-BB, epidermal growth factor (EGF) or insulin-like growth factor (IGF) elicited a dose- and time-dependent downmodulation that was reversible after transfer of SMC to growth factor-free medium. Our results support the hypothesis that T-cadherin may function as a negative determinant of cell growth.

【 授权许可】

Unknown