FEBS Letters | |
Density‐ and proliferation status‐dependent expression of T‐cadherin, a novel lipoprotein‐binding glycoprotein: a function in negative regulation of smooth muscle cell growth? | |
Kern, Frances2  Resink, Thérèse J2  Tkachuk, Vsevolod A1  Kuzmenko, Yelena S2  Bochkov, Valery N1  | |
[1] Laboratory for Molecular Endocrinology, Cardiology Research Center, Moscow, Russia;Department of Research, Laboratory for Cardiovascular Research, Basel University Hospital, CH 4031 Basel, Switzerland | |
关键词: T-cadherin; Low density lipoprotein; Smooth muscle cell; LDL; low density lipoprotein; SMC; vascular smooth muscle cell; LPDS; lipoprotein-deficient serum; FCS; fetal calf serum; SF; serum-free; PDGF; platelet-derived growth factor; EGF; epidermal growth factor; IGF; insulin-like growth factor; TBS; Tris-buffered saline; HRP; horseradish peroxidase; ECL; enhanced chemiluminescence; | |
DOI : 10.1016/S0014-5793(98)00977-6 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
The atypical low density lipoprotein (LDL) binding proteins (M r 105 and 130 kDa; p105 and p130) in human aortic medial membranes and cultured human and rat aortic smooth muscle cells (SMC) have recently been identified as the cell adhesion glycoprotein T-cadherin. Although cadherins are generally recognized to be important regulators of morphogenesis, the function of T-cadherin in the vasculature is poorly understood. This study has examined the relationship between expression of T-cadherin and the density and proliferation status of SMC. T-cadherin (p105 and p130) levels in SMC lysates were measured on Western blots using ligand-binding techniques. T-cadherin expression was dependent upon cell density, and maximal levels were achieved at confluency. T-cadherin levels were reversibly modulated by switching cultures between serum-free (upmodulation) and serum-containing (downmodulation) conditions. Platelet-derived growth factor (PDGF)-BB, epidermal growth factor (EGF) or insulin-like growth factor (IGF) elicited a dose- and time-dependent downmodulation that was reversible after transfer of SMC to growth factor-free medium. Our results support the hypothesis that T-cadherin may function as a negative determinant of cell growth.
【 授权许可】
Unknown
【 预 览 】
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RO201912020306450ZK.pdf | 209KB | download |