FEBS Letters | |
Dimeric and tetrameric forms of catalytically active transmembrane CD38 in transfected HeLa cells | |
Zocchi, Elena2  Guida, Lucrezia2  Franco, Luisa2  Bruzzone, Santina2  Corte, Giorgio1  De Flora, Antonio2  | |
[1] National Institute for Cancer Research-CBA, University of Genoa, Genoa, Italy;Institute of Biochemistry, University of Genoa, Viale Benedetto XV, 1, 16132 Genoa, Italy | |
关键词: CD38; Cyclic ADP-ribose; CD38 oligomer; Ectoenzyme; NAD+; cADPR; cyclic ADP-ribose; NAD+; nicotinamide adenine dinucleotide; ADPR; ADP-ribose; BS3; bis(sulfosuccinimidyl)suberate; rCD38; recombinant soluble CD38; β-OG; β-octylglucopyranoside; PMSF; phenylmethylsulfonyl fluoride; PBS; phosphate-buffered saline; NHD+; nicotinamide hypoxanthine dinucleotide; ϵ-NAD+; 1-N 6-etheno NAD+; ϵ-ADPR; 1-N 6-etheno ADP-ribose; cIDPR; cyclic inosine diphosphoribose; IDPR; inosine diphosphoribose; M r; molecular weight; mAb; monoclonal antibody; GSH; glutathione (reduced form); | |
DOI : 10.1016/S0014-5793(98)00929-6 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
CD38, a type II transmembrane glycoprotein, behaves as a catalytically active transporter responsible for ectocellular generation of cyclic ADP-ribose (cADPR) from NAD+ and for subsequent influx of cADPR across membranes [Franco, L., Guida, L., Bruzzone, S., Zocchi, E., Usai, C. and De Flora, A. (1998) FASEB J. in press]. cADPR regulates intracellular calcium homeostasis by releasing calcium from responsive stores. The cADPR-transporting function of CD38 requires channel-generating oligomeric forms of the protein rather than the 46 kDa monomers that have been described so far in CD38+ cells. Here we demonstrate that CD38, both in reconstituted proteoliposomes and in CD38-transfected HeLa cells, is a mixture of catalytically active monomers, homodimers and homotetramers. A soluble recombinant form of CD38 corresponding to its ectocellular region proved to be monomeric. Thus, association of native CD38 with wither artificial or natural membranes seems to result in a reversible juxtaposition of monomers suitable to cADPR-transporting activity.
【 授权许可】
Unknown
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