Brain‐specific gene expression by immortalized microglial cell‐mediated gene transfer in the mammalian brain

FEBS Letters

Hayakawa, Motoharu¹  Nagatsu, Toshiharu²  Suzuki, Hiromi¹  Kanno, Tetsuo¹  Sawada, Makoto²  Imai, Fumihiro¹ 

[1] Department of Neurosurgery, Fujita Health University, Toyoake, Aichi 470-11, Japan;PRESTO, Japan Science and Technology Corporation, Fujita Health University, Toyoake, Aichi 470-11, Japan

关键词: Microglia;    Microglial cell line;    Gene transfer;    Brain-specific;    Drug delivery system;   

DOI  :  10.1016/S0014-5793(98)00879-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The intra-arterial injection of immortalized microglia transfected with the lacZ gene, resulted in the expression of β-galactosidase in the rat brain at 48 h and the activity of β-galactosidase was detected for up to 3 weeks post-injection. More than 30-fold higher activity of β-galactosidase was detected in the brain than in the liver, lung or spleen at 48 h post-injection. This method allows us to easily deliver the gene of interest to the brain without influencing other organs. Our brain-targeting gene delivery system can facilitate gene therapy of several brain disorders, including brain tumor, metabolic disorders, and degenerative disorders, as well as investigation into the roles of particular genes in brain function and development.

【 授权许可】

Unknown