Mitsugumin23, a novel transmembrane protein on endoplasmic reticulum and nuclear membranes

FEBS Letters

Takeshima, Hiroshi³  Iino, Masamitsu³  Kangawa, Kenji¹  Komazaki, Shinji²  Nishi, Miyuki³ 

[1] Department of Biochemistry, National Cardiovascular Center Research Institute, Suita, Osaka 565-8565, Japan;Department of Anatomy, Saitama Medical School, Moroyama-machi, Saitama 350-0495, Japan;Department of Pharmacology, Faculty of Medicine, University of Tokyo, Hongo, Bunkyo-ku, and CREST, Japan Science and Technology, Tokyo 113-8654, Japan

关键词: Endoplasmic reticulum;    Sarcoplasmic reticulum;    Nuclear membrane;    cDNA cloning;    Monoclonal antibody;    Immunohistochemistry;    CHO;    Chinese hamster ovary;    ER;    endoplasmic reticulum;    E-C coupling;    excitation-contraction coupling;    mAb;    monoclonal antibody;    PAGE;    polyacrylamide gel electrophoresis;    SR;    sarcoplasmic reticulum;    T-tubule;    transverse tubule;   

DOI  :  10.1016/S0014-5793(98)00864-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We report the identification using monoclonal antibody and the primary structure by cDNA cloning of mitsugumin23, a novel transmembrane protein with a molecular mass of ∼23 kDa from skeletal muscle sarcoplasmic reticulum. Mitsugumin23 possesses three putative transmembrane segments, and its carboxy-terminal hydrophilic region exhibits sequence similarity with the tail-end portion of the myosin heavy chain. Immunochemical analysis showed that this protein is distributed throughout the outer nuclear membrane and the sarcoplasmic reticulum including the terminal cisternae at the triad junction in skeletal muscle cells. Furthermore, RNA blotting and immunohistochemical experiments demonstrated that mitsugumin23 is distributed among a wide variety of cell types in various tissues. The distribution and primary structure indicate the possibility that mitsugumin23 interacts with cytoplasmic protein(s) and participates in a housekeeping function on the intracellular organelle membranes.

【 授权许可】

Unknown