A large conductance ion channel in the nuclear envelope of a higher plant cell

FEBS Letters

Weiger, T.¹  Matzke, A.J.M.¹  Behensky, C.¹  Matzke, M.A.¹ 

[1] Institute of Molecular Biology, Austrian Academy of Sciences, Billrothstrasse 11, A-5020 Salzburg, Austria

关键词: Coconut endosperm;    Ion channel;    Nuclear envelope;    Nuclear membrane;    Nuclear pore;    Patch clamp;   

DOI  :  10.1016/0014-5793(92)80290-W

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

To detect and characterize ion channel activity in the nuclear envelope of a higher plant cell, we performed patch clamp experiments on nuclei isolated from coconut endosperm cells and on giant liposomes containing nuclear envelope fragments prepared from the same cells. An ion channel exhibiting a number of conductance substates, with a maximum of ca. 1,000 pS, was observed. Above an applied potential of ± 100 mV, the behavior of the channel was similar in isolated nuclei and liposomes, indicating that both patch clamp modes were detecting the same channel. That such a channel has now been identified in members of both the animal and plant kingdoms reinforces the notion that the nuclear pores are not always open to ions.

【 授权许可】

Unknown