FEBS Letters | |
Clofibrate‐inducible, 28‐kDa peroxisomal integral membrane protein is encoded by PEX11 | |
Fujiki, Yukio1  Abe, Isao1  Okumoto, Kanji1  Tamura, Shigehiko1  | |
[1] Department of Biology, Faculty of Science, Kyushu University, Fukuoka 812-8581, Japan | |
关键词: Peroxisome biogenesis; Peroxin; Dilysine motif; Membrane protein; Clofibrate; Complementation group; CG; complementation group; EST; expressed sequence tag; PEX11; cDNA encoding the peroxin Pex11p; PMP28; 28-kDa peroxisomal membrane protein; PTS1; peroxisome targeting signal type 1; | |
DOI : 10.1016/S0014-5793(98)00815-1 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
We cloned a human PEX11 cDNA by expressed sequence tag homology search using yeast Candida boidinii PEX11, followed by screening of human liver cDNA library. PEX11 encoded a peroxisomal protein Pex11p comprising 247 amino acids, with two transmembrane segments and a dilysine motif at the C-terminus. Pex11p comigrated in SDS-PAGE with a 28-kDa peroxisomal integral membrane protein (PMP28) isolated from the liver of clofibrate-treated rats and was crossreactive to anti-PMP28 antibody, thereby indicating PEX11 to encode PMP28. Pex11p exposes both N- and C-terminal parts to the cytosol. PEX11 was not responsible for ten complementation groups of human peroxisome deficiency disorders.
【 授权许可】
Unknown
【 预 览 】
Files | Size | Format | View |
---|---|---|---|
RO201912020306292ZK.pdf | 321KB | download |