Clofibrate‐inducible, 28‐kDa peroxisomal integral membrane protein is encoded by PEX11

FEBS Letters

Fujiki, Yukio¹  Abe, Isao¹  Okumoto, Kanji¹  Tamura, Shigehiko¹ 

[1] Department of Biology, Faculty of Science, Kyushu University, Fukuoka 812-8581, Japan

关键词: Peroxisome biogenesis;    Peroxin;    Dilysine motif;    Membrane protein;    Clofibrate;    Complementation group;    CG;    complementation group;    EST;    expressed sequence tag;    PEX11;    cDNA encoding the peroxin Pex11p;    PMP28;    28-kDa peroxisomal membrane protein;    PTS1;    peroxisome targeting signal type 1;   

DOI  :  10.1016/S0014-5793(98)00815-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We cloned a human PEX11 cDNA by expressed sequence tag homology search using yeast Candida boidinii PEX11, followed by screening of human liver cDNA library. PEX11 encoded a peroxisomal protein Pex11p comprising 247 amino acids, with two transmembrane segments and a dilysine motif at the C-terminus. Pex11p comigrated in SDS-PAGE with a 28-kDa peroxisomal integral membrane protein (PMP28) isolated from the liver of clofibrate-treated rats and was crossreactive to anti-PMP28 antibody, thereby indicating PEX11 to encode PMP28. Pex11p exposes both N- and C-terminal parts to the cytosol. PEX11 was not responsible for ten complementation groups of human peroxisome deficiency disorders.

【 授权许可】

Unknown