期刊论文详细信息
FEBS Letters | |
Antibodies against Pex14p block ATP‐independent binding of matrix proteins to peroxisomes in vitro | |
Oh, Jaesung1  Baker, Alison1  Lopez-Huertas, Eduardo1  | |
[1] Centre for Plant Sciences, Leeds Institute of Plant Biotechnology and Agriculture, University of Leeds, Leeds LS2 9JT, UK | |
关键词: Peroxisomal protein import; Peroxin; Pex14p; Peroxisome biogenesis; In vitro protein translocation assay; Sunflower glyoxysome; GO; glycolate oxidase; MDH; malate dehydrogenase; PMP22; 22 kDa peroxisomal membrane protein; AtPex14; Arabidopsis thaliana Pex14; HsPex14; human Pex14; PTS1 and PTS2; peroxisome targeting signal types 1 and 2; | |
DOI : 10.1016/S0014-5793(99)01239-9 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
The membrane protein Pex14p is a key component of the protein import machinery of peroxisomes. Antibodies raised against human Pex14p recognise a 66 kDa protein in sunflower glyoxysomes (HaPex14p) and immunoprecipitate in vitro-translated Arabidopsis Pex14p (AtPex14p). These antibodies inhibit the ATP-independent binding to sunflower peroxisome membranes of peroxisome targeting signal type (PTS) 1- and PTS2-targeted matrix proteins, but not an integral membrane protein. These results suggest that Pex14p functions before the ATP-dependent step of peroxisome assembly.
【 授权许可】
Unknown
【 预 览 】
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