Antibodies against Pex14p block ATP‐independent binding of matrix proteins to peroxisomes in vitro

FEBS Letters

Oh, Jaesung¹  Baker, Alison¹  Lopez-Huertas, Eduardo¹ 

[1] Centre for Plant Sciences, Leeds Institute of Plant Biotechnology and Agriculture, University of Leeds, Leeds LS2 9JT, UK

关键词: Peroxisomal protein import;    Peroxin;    Pex14p;    Peroxisome biogenesis;    In vitro protein translocation assay;    Sunflower glyoxysome;    GO;    glycolate oxidase;    MDH;    malate dehydrogenase;    PMP22;    22 kDa peroxisomal membrane protein;    AtPex14;    Arabidopsis thaliana Pex14;    HsPex14;    human Pex14;    PTS1 and PTS2;    peroxisome targeting signal types 1 and 2;   

DOI  :  10.1016/S0014-5793(99)01239-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The membrane protein Pex14p is a key component of the protein import machinery of peroxisomes. Antibodies raised against human Pex14p recognise a 66 kDa protein in sunflower glyoxysomes (HaPex14p) and immunoprecipitate in vitro-translated Arabidopsis Pex14p (AtPex14p). These antibodies inhibit the ATP-independent binding to sunflower peroxisome membranes of peroxisome targeting signal type (PTS) 1- and PTS2-targeted matrix proteins, but not an integral membrane protein. These results suggest that Pex14p functions before the ATP-dependent step of peroxisome assembly.

【 授权许可】

Unknown