Trypanosoma brucei tryparedoxin, a thioredoxin‐like protein in African trypanosomes

FEBS Letters

Krauth-Siegel, R.Luise²  Stallmann, Dirk¹  Lüdemann, Heike²  Dormeyer, Matthias²  Follmann, Hartmut¹  Sticherling, Christian² 

[1] Fachbereich Biologie/Chemie, Universität Gesamthochschule Kassel, D-34132 Kassel, Germany;Biochemie-Zentrum Heidelberg, Ruprecht-Karls-Universität, Im Neuenheimer Feld 328, D-69120 Heidelberg, Germany

关键词: Tryparedoxin;    Thioredoxin;    CPPC motif;    Trypanothione;    Trypanosoma brucei;    tpx;    gene encoding tryparedoxin;    TR;    trypanothione reductase (EC 1.6.4.8);    T(SH)2;    trypanothione [N 1;    N 8-bis(glutathionyl)spermidine];    TS2;    trypanothione disulfide;    GSH;    glutathione;    GSSG;    glutathione disulfide;    HEDS;    β-hydroxyethyl disulfide;   

DOI  :  10.1016/S0014-5793(98)00793-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A gene has been cloned from Trypanosoma brucei which encodes a protein of 144 amino acid residues containing the thioredoxin-like motif WCPPCR. Overexpression of the gene in E. coli resulted in 4 mg pure protein from 100 ml bacterial cell culture. Recombinant T. brucei tryparedoxin acts as a thiol-disulfide oxidoreductase. It is spontaneously reduced by trypanothione. This dithiol, exclusively found in parasitic protozoa, also reduces E. coli glutaredoxin but not thioredoxin. The trypanothione/tryparedoxin couple is an effective reductant of T. brucei ribonucleotide reductase. Like thioredoxins it has a poor GSH:disulfide transhydrogenase activity. The catalytic properties of tryparedoxin are intermediate between those of classical thioredoxins and glutaredoxins which indicates that these parasite proteins may form a new class of thiol-disulfide oxidoreductases.

【 授权许可】

Unknown