Evidence for gene duplication forming similar binding folds for NAD(P)H and FAD in pyridine nucleotide‐dependent flavoenzymes

FEBS Letters

McKie, James H.¹  Douglas, Kenneth T.¹ 

[1] Department of Pharmacy, University of Manchester, Manchester, M13 9PL, UK

关键词: Flavoenzyme;    Gene duplication;    Glutathione;    Lippamide;    Reductase;    Trypanothione;   

DOI  :  10.1016/0014-5793(91)80236-V

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

For pyridine nucleotide-dependent flavoezymes, binding both FAD and NAD(P)H on a single amino-acids chain, we have found a high degree of internal sequence similarity for certain regions of FAD and NAD (P)H binding portions of the chain for any given protein. This was the case for a range of enzymes classes, including disulphide oxidoreductases (such as glutathione reductase, trypanothione reductase, lipoamide dehydrogenase, mercuric reductase), mono- and dioxygenases, nitrite reductaSE, alkyl hydroperoxidase and NADH dehydrogenase from E. coli. This provides strong support for gene duplication as the origin of at least part of the FAD and NAD(P)H recognising domains of such enzymes.

【 授权许可】

Unknown